Enzyme-linked immunosorbent assay (ELISA) is used to detect the presence of antibodies or antigen in a sample. In a direct ELISA, antigen is coated on the surface of plastic wells. Serum containing antibodies against this antigen are added to the wells. Labeled anti-Ig antibody is added to the wells to bind with unlabeled antibody, followed by a reaction with the appropriate substrate that changes the color of the product. This assay can also be modified for the detection of cytokines or other proteins.
E7-specific antibody responses in C57BL/6 mice immunized with various recombinant DNA vaccines. C57BL/6 mice were immunized with control plasmid (no insert), wild-type HSP70, wild-type E7, or E7-HSP70 DNA via a gene gun. Serum samples were obtained from immunized mice 14 days after vaccination. The presence of the E7-specific antibody was detected by ELISA using serial dilution of sera. The results from the 1:80 dilution are presented showing the mean absorbance (A450 nm) ± SE. (Enhancement of DNA Vaccine Potency by Linkage of Antigen Gene to an HSP70 Gene)
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