TECHNIQUES

The Gene Gun is used for the transfer of genes into living organisms. The gun uses low-pressure helium to eject cartridges of gold nano-particles into the desired object. 12 DNA or RNA-coated gold cartridges can be inserted into the holder. The Helios Gene Gun © is able to precipitate the same reaction varieties of different nucleic acids together into the subject. This enables different types of plasmids to be transferred at the same time in one experiment and thus deliver plasmids carrying various traits all in one experiment. The ability to modify the genetic makeup of cells through gene transfer can help us gain a broader understanding of gene therapy. Also, small amounts of DNA are required, thus eliminating the need for protein purification. Visit the Bio-Rad website for more information.