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The Genetics of Pancreatic Cancer

-- Technical Section

 
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BRCA2 Primers

PCR Primers
BR2TNT: 5’-GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGCAACATTTTTTGAAATTTTTAAG-3’
BR2TNTAS: 5’-GGATCAGTATCATTTGGTTC-3’
(BR2TNT/ BR2TNTAS amplified exons 2 to part of exon 10)
BR10TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGATATTAATGTRGCTTCTGTTTTATAC-3’
BR10TNTAS: 5’-GTGCTTCAAAGCATTTGCTTC-3’
BR111TNT: 5’-GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC ATGGTTTGTCACTTTGTGTTTTTTATG-3’
BR111TNTAS: 5’-GAGTTCTTGAAAATGGGTTC-3’
BR112TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGCTAATGAAAGGAATAATCTTG-3’
BR112TNTAS: 5’-AACCTCACTAATATTCTCAATATC-3’
BR113TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGTAATGAATGCCCCATCGATTG-3’
BR113TNTAS: 5’-CCCTGGAAGGTCACTAGTTG-3’
BR114TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGCTGAATTACATTCTGACATAAG-3’
BR114TNTAS: 5’-TGTGGGTATGCATTTGCATC-3’
BR115TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGCTGGTATTGAGCCAGTATTG-3’
BR115TNTAS: 5’-TTCCACTTGCTGTACTAAATC-3’
BR115.1: 5’-AGTATAGGGAAGCTTCATAAG-3’
BR115.1AS: 5’- AAAGACTTGCTTGGTACTATC-3’
BR116TNT2: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGG GTATAGGGAAGCTTCATAAG-3’
BR116TNT2AS: 5’- CTTTTTCCAATTCTTGAATTTG-3’
BR12TNT2: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGG GAACCAAAGTCTCACTTGTTG-3’
BR12TNT2AS: 5’-GCTAAGAGGGGAGGATCTAAC-3’
(BR12TNT2/ BR12TNT2AS amplified the end of exon 11 to exon 17)
BR17TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGCAGCTGCAAAAACACTTGTTC-3’
BR17TNTAS: 5’-AAAACTTTATTGCCAGTAAATTG-3’
(BR17TNT/ BR17TNTAS amplified exon 17 to exon 22)
BR22TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGGTCCATCATCAGATTTATATTC-3’
BR22TNTAS: 5’-CCCCTTTACAAGACTTTGAAG-3’
(BR22TNT/ BR22TNTAS amplified exon 22 to exon 27)
BR27TNT: 5’- GGA TCC TAA TAC GAC TCA CTA TAG GGA GAC CAC CATGGCGTTTTCATTTTTTTATCAGATG-3’
BR27TNTAS: 5’-GCTTATTTTTCTCACATTCTTC-3’
Sequencing Primers
BR115.1seq: 5’-CTATTTCAGAAAACACTTGTCTTG-3’
BR111seq: 5’-CTTGTCTGACATTTTGTATGATTC-3’

Legend to the names of the BRCA2 primers.
All primers are designated as "BR" short for the BRCA2 gene. The sense PCR primers were also designated as "TNT" if they were used to generate protein products by the in-vitro truncation assay. "AS" was used to indicate an antisense primers. The number after BR was used to indicate which exon was amplified. A subsequent number after the exon number eg. 111 was used to designate the first of a series of PCR products generated from exon 11. Where a primer pair amplified several exons, the exons amplified are indicated below the corresponding primer pair above.