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The Genetics of Pancreatic Cancer

-- Technical Section

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DPC4 Primers

EXON 1

5’ END, DPC4S1, CGTTAGCTGTTGTTTTTCACTG

3’ END, DPC4AS1, AGAGTATGTGAAGAGATGGAG

Seq primer, DPC4Ex1, TTTCCAAAGGATCAAAATTGCTTCAG

EXON 2

5’ END, DPC4S2, TGTATGACATGGCCAAGTTAG

3’ END, DPC4AS2, CAATACTCGGTTTTAGCAGTC

Seq primer, DPC4Ex2, TAATACTGAGTTGGTAGGATTGTGAG

EXON 3

5’ END, DPC4S3, CTGAATTGAAATGGTTCATGAAC

3’ END, DPC4AS3, GCCCCTAACCTCAAAATCTAC

Seq primer, DPC4Ex3, CTCACACAAACTAATTCTAGGTCAAC

EXON 4

5’ END, DPC4S4, TTTTGCTGGTAAAGTAGTATGC

3’ END, DPC4AS4, CTATGAAAGATAGTACAGTTAC

Seq primer, DPC4Ex4, GGAGTTTCCCCCCAAGTGACTAC

EXONs 5-6

5’ END, DPC4S5/6, CATCTTTATAGTTGTGCATTATC

3’ END, DPC4AS5/6 TAATGAAACAAAATCACAGGATG

Seq primer, exon 5, DPC4Ex5, TGAAATCATAAGATGACATCTATGAATG

Seq primer, exon 6, DPC4Ex6/1, CTGGACTGGAAGTAGGACTG and

      DPC4Ex6/2, TCCGGGATGGGGCGGCATAG

EXON 7

5’ END, DPC4S7, TGAAAGTTTTAGCATTAGACAAC

3’ END, DPC4AS7, TGTACTCATCTGAGAAGTGAC

Seq primer, DPC4Ex7, CACTAAATCAATCTAAATACAGGAAATC

EXON 8

5’ END, DPC4S8, TGTTTTGGGTGCATTACATTTC

3’ END, DPC4AS8, CAATTTTTTAAAGTAACTATCTGA

Seq primer, DPC4Ex8, TGTGTTGTGGAGTGCAAGTGAAAG

EXON 9

5’ END, DPC4S9, TATTAAGCATGCTATACAATCTG

3’ END, DPC4AS9, CTTCCACCCAGATTTCAATTC

Seq primer, DPC4Ex9, TTTTGACAACAAATAGAGCTTTAAGTC

EXON 10

5’ END, DPC4S10, AGGCATTGGTTTTTAATGTATG

3’ END, DPC4AS10, CTGCTCAAAGAAACTAATCAAC

Seq primer, DPC4Ex10, GAATTTTCTTTATGAACTCATAG

EXON 11

5’ END, DPC4S11, CCAAAAGTGTGCAGCTTGTTG

3’ END, DPC4AS11, CAGTTTCTGTCTGCTAGGAG

Seq primer, DPC4Ex11, TATTTTGTAGTCCACCATC