Primer sequence for Bisulfite-Sequence |
Link to MSP Primer | |||

Annealing Temp. | ||||

RARß | Forward | 5'-GAGTTGGTGATGTTAGATTAG-3' | ||

Reverse | 5'-TTCCCAAAAAAATCCCAAATTC-3' | 56 | ||

Sequence | 5'-CTCCTTCCAAATAAATACTTAC-3' | |||

THBS1 | Forward | 5'-AGAGAGGAGTTTAGATTGG-3' | ||

Reverse | 5'-CAAAAAAACTAAAACCTCAAC-3' | 54 | ||

Sequence | Forward primer | |||

CACNA1G | Forward | 5'-TGGATAAAGGATGTTTGGGGTTTG-3' | ||

Reverse | 5'-CCCTCCCCTTACCCCTAAATCC-3' | 55, 53, 51, 49* | ||

Sequence | 5'-ACTCCCCCTCACTTTATTC-3' | |||

hMLH1 | Forward | 5'-ATTATTTTAGTAGAGGTATATAAG-3' | ||

Reverse | 5'-CCAACCCCACCCTTCAAC-3' | 58 | ||

Sequence | Forward primer | |||

MINT1 | Forward | 5'-AAGAGAGGGTTGGAGAGTAG-3' | ||

Reverse | 5'-CCCCTAAAAAAAAAATCAAAAATC-3' | 62 | ||

Sequence | 5'-GGGTTGGAGAGTAGGGGAGTT-3' | |||

MINT2 | Forward | 5'-YGTTATGATTTTTTTGTTTAGTTAAT-3' | ||

Reverse | 5'-TACACCAACTACCCAACTACCTC-3' | 60, 58, 56, 54** | ||

Sequence | 5'-ACTTCCATTAAAAACAACTAC-3' | |||

MINT31 | Forward | 5'-TTTATTTATATAATTTTGTGTATGG-3' | ||

Reverse | 5'-CACCCCTCACTTTACTAAAAC-3' | 58 | ||

Sequence | Reverse primer | |||

MINT32 | Forward | 5'-TTTGGGAGGTAAATTYGTTGATT-3' | ||

Reverse | 5'-ACCRAACAAAAAACCTAAAAAAAC-3' | 58, 56, 54, 52*** | ||

Sequence | Forward primer | |||

* 55 (5 cycles), 53 (5 cycles), 51 (5 cycles), 49 (26 cycles) | ||||

** 60 (3 cycles), 58 (4 cycles), 56 (5 cycles), 54 (26 cycles) | ||||

*** 58 (3 cycles), 56 (4 cycles), 54 (5 cycles), 52 (26 cycles) |

Primer sequence for MSP | ||||

Annealing Temp. | ||||

p16 | UnMethylated | F | 5'-TTATTAGAGGGTGGGGTGGATTGT-3' | 60 |

R | 5'-CAACCCCAAACCCACAACCATAA-3' | |||

Methylated | F | 5'-TTATTAGAGGGTGGGGCGGATCGC-3' | 65 | |

R | 5'-GACCCCCGAACCGCGACCCTAA-3' | |||

RARß | UnMethylated | F | 5'-AGGATTGGGATGTTGAGAATG-3' | 58 |

R | 5'-TTACAAAAAACCTTCCAAATACA-3' | |||

Methylated | F | 5'-GGATTGGGATGTCGAGAAC-3' | 64 | |

R | 5'-TACAAAAAACCTTCCGAATACG-3' | |||

CACNA1G | UnMethylated | F | 5'-GTTTTTTTTTGGATTTTTGTTTTTTG-3' | 60 |

R | 5'-TTTATTCCAACTTCTTCACTTCA-3' | |||

Methylated | F | 5'-GTTTTTTCGGGGCGGTTTC-3' | 62 | |

R | 5'-TTCCGACTTCTTCGCTTCG-3' | |||

TIMP-3 | UnMethylated | F | 5'-TTTTGTTTTGTTATTTTTTGTTTTTGGTTTT-3' | 59 |

R | 5'-CCCCCCAAAAACCCCACCTCA-3' | |||

Methylated | F | 5'-CGTTTCGTTATTTTTTGTTTTCGGTTTTC-3' | 59 | |

R | 5'-CCGAAAACCCCGCCTCG-3' | |||

THBS1 | UnMethylated | F | 5'-GTTTGGTTGTTGTTTATTGGTTG-3' | 62 |

R | 5'-CCTAAACTCACAAACCAACTCA-3' | |||

Methylated | F | 5'-TGCGAGCGTTTTTTTAAATGC-3' | 62 | |

R | 5'-TAAACTCGCAAACCAACTCG-3' | |||

hMLH1 | UnMethylated | F | 5'-TTAATAGGAAGAGTGGATAGTG-3' | 56 |

R | 5'-TCTATAAATTACTAAATCTCTTCA-3' | |||

Methylated | F | 5'-TTAATAGGAAGAGCGGATAGC-3' | 58 | |

R | 3'-CTATAAATTACTAAATCTCTTCG-3' | |||

E-Cad | UnMethylated | F | 5'-TAATTTTAGGTTAGAGGGTTATTGT-3' | 53 |

R | 5'-CACAACCAATCAACAACACA-3' | |||

Methylated | F | 5'-TTAGGTTAGAGGGTTATCGCGT-3' | 57 | |

R | 5'-TAACTAAAAATTCACCTACCGAC-3' | |||

DAPK | UnMethylated | F | 5'-GGAGGATAGTTGGATTGAGTTAATGTT-3' | 60 |

R | 5'-CAAATCCCTCCCAAACACCAA-3' | |||

Methylated | F | 5'-GGATAGTCGGATCGAGTTAACGTC-3' | 60 | |

R | 5'-CCCTCCCAAACGCCGA-3' | |||

MGMT | UnMethylated | F | 5'-TTTGTGTTTTGATGTTTGTAGGTTTTTGT-3' | 59 |

R | 5'-AACTCCACACTCTTCCAAAAACAAAACA-3' | |||

Methylated | F | 5'-TTTCGACGTTCGTACCTTTTCGC-3' | 59 | |

R | 5'-GCACTCTTCCGAAAACGAAACG-3' | |||

MINT1 | UnMethylated | F | 5'-GGGGTTGAGGTTTTTTGTTAGT-3' | 64 |

R | 5'-TTCACAACCTCAAATCTACTTCA-3' | |||

Methylated | F | 5'-GGGTTGAGGTTTTTTGTTAGC-3' | 64 | |

R | 5'-CTACTTCGCCTAACCTAACG-3' | |||

MINT2 | UnMethylated | F | 5'-GGTGTTGTTAAATGTAAATAATTTG-3' | 58 |

R | 5'-AAAAAAAAACACCTAAAACTCA-3' | |||

Methylated | F | 5'-AATCGAATTTGTCGTCGTTTC-3' | 60 | |

R | 5'-AAATAAATAAATAAAAAAAAACGCG-3' | |||

MINT31 | UnMethylated | F | 5'-GAATTGAGATGATTTTAATTTTTTGT-3' | 64 |

R | 5'-CTAAAACCATCACCCCTAAACA-3' | |||

Methylated | F | 5'-TTGAGACGATTTTAATTTTTTGC-3' | 62 | |

R | 5'-AAAACCATCACCCCTAAACG-3' | |||

MINT32 | UnMethylated | F | 5'-GAGTGGTTAGAGGAATTTAGGT-3' | 62 |

R | 5'-CTAAAAAAACAAACAAAACATCCA-3' | |||

Methylated | F | 5'-GTGGTTAGAGGAATTTAGGC-3' | 64 | |

R | 5'-AAAACGAACGAAACGTCCG-3' | |||