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The assessment of protective immunity conferred by vaccination
has three essential steps. First, an immune response is elicited by immunization
with a candidate vaccine. Second, the immunized mice, along with unimmunized
controls, are challenged with antigen-expressing tumors. Finally, the antitumor
effects in the immunized mice and unimmunized controlsis are compared.
Vaccination with E7-HSP70 DNA protects mice against the growth
of TC-1 tumors.C57BL/6 mice were immunized with empty plasmid (pcDNA3),
HSP70 DNA (HSP), E7 DNA (E7), or E7-HSP70 DNA (E7/HSP) via a gene gun. One week
after the last vaccination, mice were challenged with 5 x 104 TC-1 cells/mouse
s.c. in the right leg. The mice were monitored for evidence of tumor growth
by palpation and inspection twice a week. A, each mouse received 2 µg
of DNA vaccine. One week later, the mice were revaccinated with the same type
and amount of DNA as the first vaccination. B, each mouse received only one
injection of 2 µg of DNA vaccine without a further booster. (Enhancement
of DNA Vaccine Potency by Linkage of Antigen Gene to an HSP70 Gene)
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