The cytotoxic T lymphocyte assay is used to detect the cytolytic activity of antigen-specific T cells. Target cells are labeled with radioactive sodium chromate, lactate dehydrogenase (LDH), or other readily detectable compound. A sample containing T cells is then added to the mixture. When these target cells are killed, the chromate or LDH is is released, providing a measure of cytotoxicity.
CTL assays. A, CTL assays to demonstrate enhanced presentation of E7 through the MHC class I pathway of cells transfected with ETA(dII)/E7 DNA. 293 Db, Kb cells transfected with various DNA constructs served as target cells. These CTL assays were performed with various E:T ratios using Db-restricted E7-specific CD8+ T cells as target cells. B, CTL assays to demonstrate enhanced
MHC class I presentation of E7 in bone marrow-derived DCs pulsed with cell lysates containing chimeric ETA(dII)/E7 protein. Bone marrow-derived DCs were pulsed with cell lysates from various DNA-transfected 293 Db, Kb cells at different concentrations as described in "Materials and Methods." These assays were performed at a fixed E:T (9:1) ratio using Db-restricted E7-specific CD8+ T cells as effector cells. (Cancer Immunotherapy Using a DNA Vaccine Encoding the Translocation Domain of a Bacterial Toxin Linked to a Tumor Antigen)
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