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MHC:peptide tetramers consist of four peptide-bound MHC complexes
adjoined to avidin or streptavidin by biotin. Flurochrome-labeled MHC:peptide
tetramers are bound by receptors on T cells and can be detected and counted
using flow cytometry analysis. This technique allows for the direct enumeration
of antigen-specific T cells by their receptor specificity, rather than through
other more indirect assays.
Flow cytometry analysis using double staining with CD8-specific
antibody and E7 peptide-pulsed Db tetramer. X-axis represents CD8 staining
an y-axis represents tetramer staining. A) E7-specific T cell clone. B) Splenocytes
from mice vaccinated with an effective E7-containing DNA vaccine. C) Splenocytes
from mice vaccinaated with control plasmid.
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