Information for the antibodies we used

Table 1. Dilutions and Sources of Antibody Panel Used in the Immunohistochemistry

Antigen	Antibody*	Dilution	Source
p63	4A4	1:100	Neomarker
Cytokeratin 18	DC10	1:10	Dako
hPL	HPL	1:6,000	Dako
Ki-67	MIB-1	1:1,000	Dako
HLA-G	MEM-G/1	1:200	abcam
hCG	hCG	1:2,000	Dako
Mel-CAM (CD146)	NCL-CD146	1:50	Novo Castra

* All the antibodies are commercially available and work on paraffin sections.

Cytokeratin 18 staining in ETT

Cytokeratin 18 staining in squamous carcinomas

Inhibin-a staining in ETT

Inhibin-a staining in squamous carcinoma

HLA-G immunoreactivity

Chorion laeve	Placental site nodule	ETT
HLA-G immunoreactivity

Trophoblastic column	Choriocarcinoma
HLA-G immunoreactivity

p63 staining in ETT

A mixed case of PSTT and ETT

Double Staining for Mel-CAM and Ki-67

The presence of proliferation activity distinguishes an EPS from a PSTT- a double staining using IT specific marker and Ki-67